Seed biopriming with Moringa oleifera leaf and bark extracts improves seed germination and early seedling vigor of rice

Authors

  • Md. Parvez Anwar
    Department of Agronomy, Bangladesh Agricultural University, Mymensingh - 2202, Bangladesh
  • Mst. Zinat Hossain
    Department of Agronomy, Bangladesh Agricultural University, Mymensingh - 2202, Bangladesh
  • Nazmun Naher Priya
    Department of Agronomy, Bangladesh Agricultural University, Mymensingh - 2202, Bangladesh
  • Md. Nazmus Sakib
    Department of Agronomy, Bangladesh Agricultural University, Mymensingh - 2202, Bangladesh
  • Swapan Kumar Paul
    Department of Agronomy, Bangladesh Agricultural University, Mymensingh - 2202, Bangladesh

DOI:

https://doi.org/10.26832/24566632.2026.110309

Keywords:

Bio-stimulant, Seedling vigor index, Stress tolerance, Water extraction method

Abstract

Seed priming is a pre-sowing treatment that promotes rapid and uniform germination, seedling establishment, and early vigor, depending on the priming agent and its concentration. This study evaluated the potentiality of Moringa extract (Moringa oleifera) as a biopriming agent for enhancing seed germination and seedling growth of rice. Water extraction method was applied for the extraction of Moringa bark and leaf extracts (MBE and MLE) followed by diluting 10, 15, 20 and 25 times. Nine priming treatments such as- control (no priming), MBE10, MBE15, MBE20, MBE25, MLE10, MLE15, MLE20 and MLE25 were used in the experiment with four replications. A clear advantage of biopriming with Moringa extract over no priming was evident for rice seed germination and seedling growth and vigor and bark extract performed better than leaf extract. In general, higher concentration resulted in better performances for most of the parameters studied. For germination parameters, the maximum germination (93.75%), shortest mean germination time (5.30) and utmost germination index (71.00) were recorded for MBE10. For seedling growth parameters, highest shoot length (15.60 cm), highest root length (11.85 cm), highest shoot dry weight (0.0483 g), highest root dry weight (0.0535 g) and highest root-shoot ratio (0.76) were also recorded from biopriming with MBE10. The findings demonstrate that Moringa bark extract has considerable potential as a seed biopriming agent for enhanced seed germination, seedling growth and vigor of rice. Further studies should characterize the bioactive compounds in Moringa extracts and elucidate the mechanisms responsible for the observed responses.

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References

Abdelhameed, R. E., Galilah, D. A., & Metwally, R. (2025). Multifaceted role of Moringa oleifera leaf extract as antimicrobial, growth enhancer and mitigator of salt stress in tomato seedlings. BMC Plant Biology, 25, 1144. https://doi.org/10.1186/s12870-025-07149-7

Adebisi, M.A. (2004). Variation, stability and correlation studies in seed quality and yield of sesame (Sesamum indicum L.). Ph.D. Thesis, University of Agriculture, Abeokuta.

Ahmed, T., Elezz, A. A., & Khalid, M. (2021). Hydropriming with Moringa leaf extract mitigates salt stress in wheat seedlings. Agriculture, 11(12), 1254. https://doi.org/10.3390/agriculture11121254

Al Khazan, M. M. (2020). Priming with Moringa (Moringa oleifera Lam.) leaf extract boosts the growth and physio-biochemical attributes of lead-stressed fenugreek (Trigonella foenum-graecum L.) seedlings. Applied Ecology and Environmental Research, 18(5), 6949–6967. https://doi.org/10.15666/aeer/1805_69496967

Anwar, F., Latif, S., Ashraf, M., & Gilani, A. (2007). Moringa oleifera: A food plant with multiple medicinal uses. Phytotherapy Research, 21, 17-25. https://doi.org/10.1002/ptr.2023

Anwar, M. P., Jahan, R., Rahman, M. R., Islam, A. K. M. M., & Uddin, F. M. J. (2021a). Seed priming for increased seed germination and enhanced seedling vigor of winter rice. IOP Conference Series: Earth and Environmental Science, 756, 012047. https://doi.org/10.1088/1755-1315/756/1/012047

Anwar, M. P., Khalid, M., Islam, A. K. M. M., Yeasmin, S., Ahmed, S., Hadifa, A., Ismail, I. A., Hossain, A., & Sabagh, A. E. (2021b). Potentiality of different seed priming agents to mitigate cold stress of winter rice seedling. Phyton- International Journal of Experimental Botany, 90(5), 1491–1506. https://doi.org/10.32604/phyton.2021.015822

Anwar, M.P., Akhter, M, Aktar, S., Kheya, S.A., Islam, A.K.M.M., Yeasmin, S., Hasan, A.K. and Rashid, M.H. (2024). Relationship between Seed Priming Mediated Seedling Vigor and Yield Performance of Spring Wheat. Phyton-International Journal of Experimental Botany, 93(6), 1159–1177. https://doi.org/10.32604/phyton.2024.049073

AOSA, Association of Official Seed Analysts. (1983). Seed vigor testing handbook (Contribution No. 32 to the Handbook on Seed Testing). Association of Official Seed Analysts.

Arif, Y., Bajguz, A., & Hayat, S. (2023). Moringa oleifera extract as a natural plant biostimulant. Journal of Plant Growth Regulation, 42(3), 1291–1306. https://doi.org/10.1007/s00344-022-10630-4

Benramadan, S. M., & Alsabri, M. A. (2025). Effect of Different concentration of the Aqueous leaf extract of Moringa olifeira on seed germination and early growth of Lycopersicon esculentum mill. African Journal of Advanced Pure and Applied Sciences, 4(4), 841–851. Retrieved from https://aaasjournals.com/index.php/ajapas/article/view/1788

El-Rokiek, K. G., Shehata, A. N., El-Din, S. A. S., & Eid, R. A. (2022). Herbicidal potential and identification of allelochemicals from Moringa oleifera. Asian Journal of Plant Sciences, 21, 154–162. https://doi.org/10.3923/ajps.2022.154.162

Ellis, R.H. and Roberts, E.H. (1981) The Quantification of Ageing and Survival in Orthodox Seeds. Seed Science and Technology, 9, 373-409.

Farooq, M., Wahid, A., Basra, S., & Siddique, K. (2019). Improving crop resistance to abiotic stresses through seed invigoration. In Handbook of Plant and Crop Stress (4th ed.). https://doi.org/10.1201/9781351104609-41

Foidl, N., Makkar, H. P. S., & Becker, K. (2001). The potential of Moringa oleifera for agricultural and industrial uses. In L. J. Fuglie (Ed.), The miracle tree: The multiple attributes of Moringa (pp. 45–76). CTA, Wageningen, The Netherlands.

Haider, N., Noor, A., Zeb, M., Abro, M., Hussain, S., Baloch, G., Khoso, A., & Wahocho, S. (2024). Evaluating the effect of priming with Moringa leaf extract on seed germination and seedling growth of bitter gourd (Momordica charantia L). Journal of Horticulture and Agricultural Sciences, 1, 53–64. https://doi.org/10.63459/jhas.2024.1.01

Harris, D., Joshi, A., Khan, P., Gothkar, P., & Sodhi, P. S. (1999). On-farm seed priming in semi-arid agriculture: Development and evaluation in maize, rice and chickpea in India using participatory methods. Experimental Agriculture, 35, 15–29. https://doi.org/10.1017/s0014479799001027

Islam, A. K. M. M., Khatun, T., Bipra, P. C., Sazzad, M., Roy, T. K., Rana, M. M., Yeasmin, S., Kheya, S. A., Urmi, S. A., Meem, M. M. M., Anwar, M. P., & Islam, A. K. M. A. (2026). Seed priming-induced enhancement in seed germination, Seedling vigor, and productivity of foxtail millet (Setaria italica L.) in winter and summer seasons under Bangladesh conditions. PLOS ONE, 21(4), e0348288. https://doi.org/10.1371/journal.pone.0348288

Jamal, M. R., Kristiansen, P., Kabir, M. J., & De Bruyn, L. L. A. (2023). Challenges and adaptations for resilient rice production under changing environments in Bangladesh. Land. https://doi.org/10.3390/land12061217

Kamanga, B. M., Cartmill, D. L., McGill, C., & Clavijo McCormick, A. (2025). Allelopathic effects of Moringa oleifera Lam. on cultivated and non-cultivated plants: Implications for crop productivity and sustainable agriculture. Agronomy, 15(8), 1766. https://doi.org/10.3390/agronomy15081766

Karmore, J. V., & Tomar, G. (2015). Effects of seed priming methods on germination and seedling development of winter maize (Zea mays L.). Advance Research Journal of Crop Improvement, 6, 88–93. https://doi.org/10.15740/has/arjci/6.2/88-93

Kaur, S., Gupta, A., & Kaur, N. (2002). Effect of osmo- and hydropriming of chickpea seeds on seedling growth and carbohydrate metabolism under water deficit stress. Plant Growth Regulation, 37, 17–22. https://doi.org/10.1023/a:1020310008830

Khan, S., Ibrar, D., Bashir, S., Rashid, N., Hasnain, Z., Nawaz, M., Al-Ghamdi, A., Elshikh, M., Dvořáčková, H., & Dvořáček, J. (2022). Application of Moringa leaf extract as a seed priming agent enhances growth and physiological attributes of rice seedlings cultivated under water deficit regime. Plants, 11, 261. https://doi.org/10.3390/plants11030261

Mamun, M. A. A., Nihad, S., Sarkar, M. A. R., Aziz, M. A., Qayum, M., Ahmed, R., Rahman, N., Hossain, M. I., & Kabir, M. S. (2021). Growth and trend analysis of area, production and yield of rice: A scenario of rice security in Bangladesh. PLoS ONE, 16. https://doi.org/10.1371/journal.pone.0261128

Nouman, W., Siddiqui, M. T., & Basra, S. M. A. (2012). Moringa oleifera leaf extract: An innovative priming tool for rangeland grasses. Turkish Journal of Agriculture, 35, 65–75. https://doi.org/10.3906/tar-1009-1261

Paparella, S., Araújo, S., Rossi, G., Wijayasinghe, M., Carbonera, D., & Balestrazzi, A. (2015). Seed priming: State of the art and new perspectives. Plant Cell Reports, 34, 1–13. https://doi.org/10.1007/s00299-015-1784-y

Parvin, S., Anwar, M. P., Kabiraj, M. S., Rashid, M. H., & Paul, S. K. (2024). Improvement of Seed Germination and Seedling Growth of Faba Bean (Vicia Faba L.) through Seed Priming. Turkish Journal of Agriculture - Food Science and Technology, 12, 561–567. https://doi.org/10.24925/turjaf.v12i4.561-567.6649

Perveen, S., Mushtaq, M., Yousaf, M., & Sarwar, N. (2020). Allelopathic hormesis and potent allelochemicals from multipurpose tree Moringa oleifera leaf extract. Plant Biosystems, 155, 154–158. https://doi.org/10.1080/11263504.2020.1727984

Phiri, C. & Mbewe (2010). Influence of Moringa oleifera leaf extracts on germination and early seedling development of major cereals. Agriculture and Biology Journal of North America, 1(5), 774–777. https://doi.org/10.5251/abjna.2010.1.5.774.777

Rahman, A. B. R. B., & Zhang, J. (2022). Trends in rice research: 2030 and beyond. Food and Energy Security. https://doi.org/10.1002/fes3.390

Roy, P.K., Sarkar, M.A.R., Paul, S.K. & Dey, A. (2013). Effect of osmopriming of Boro rice seeds on field establishment of seedlings. International Journal of Applied Science and Biotechnology, 1(4), 228-232.

Sarmin, T., Islam, M. F., Hossain, M. S., Kader, M. A., Hasan, A. K., Islam, A. K. M. M., & Anwar, M. P. (2025). Improving Winter Rice Establishment through Seed Priming. Asian Research Journal of Agriculture, 18(3), 294-305. https://doi.org/10.9734/arja/2025/v18i3737

Soares, T. F. S. N., da Silva, A. V. C., & Muniz, E. N. (2021). Moringa leaf extract: A cost-effective and sustainable product to improve plant growth. South African Journal of Botany, 141, 171–176. https://doi.org/10.1016/j.sajb.2021.04.007

Yasmeen, A., Basra, S., Wahid, A., Nouman, W., & Rehman, H. (2013). Exploring the potential of Moringa oleifera leaf extract (MLE) as a seed priming agent in improving wheat performance. Turkish Journal of Botany. 37 (3), 512-520. https://doi.org/10.3906/bot-1205-19

Yasmeen, A., Wasif, B., Bullet, N., Maqsood, S., Basra, A., Wahid, A., Hafeez-Ur-Rehman, B., Nazim, B., Bullet, H., & Afzal, I. (2014). Morphological and physiological response of tomato (Solanum lycopersicum L.) to natural and synthetic cytokinin sources: A comparative study. Acta Physiologiae Plantarum, 36, 3147–3155. https://doi.org/10.1007/s11738-014-1662-1

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Published

25-09-2026

How to Cite

Anwar, M. P., Hossain, M. Z., Priya, N. N., Sakib, M. N., & Paul, S. K. (2026). Seed biopriming with Moringa oleifera leaf and bark extracts improves seed germination and early seedling vigor of rice. Archives of Agriculture and Environmental Science, 11(3), 333–338. https://doi.org/10.26832/24566632.2026.110309

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Research Articles