Effects of organic fertilizers on the macropropagation, vegetative growth, and pest infestation on plantain (Musa sp.)
Abstract
One of the major constraints to the expansion of plantain cultivation has been the scarcity of healthy planting material. Decapitation, following macropropagation technology, stimulation of lateral bud development and plantlet production. This study aimed to evaluate the efficacy of decapitation, following macropropagation technology, to stimulate lateral bud development and plantlet production in Musa sp. The experiment was carried out at both the macropropagators' level and in the nursery. The experiment was laid out using the Randomized Complete Block Design (RCBD) with five treatments (Control, T1 – Biozyme, T2 – Radix Tim, T3 – Compo, and T4 – DI Grow) with 10 replicates in three blocks. All plants were administered equal amounts of each treatment. The results at the bud incubation level revealed that bud initiation was not significantly dependent on the treatment. Although not significant, the Biozyme® treatment had the highest mean number of plantlets (46 plants). The organic fertilizers had significant effects (p = 0.001) on the vegetative growth of nursery plants. The maximum vegetative growth (plant height of 7.86±0.32 cm, girth of 10.6±0.3cm, 4±0.12 number of leaves, leaf area of 144 ±5.00 cm2) was recorded with plants treated with T2 – Radix Tim® (50 ml/7.5 L of water). Snails were the only nursery pests identified, and plants treated with T3 (Compo®) were more susceptible to snails, with a mean of 0.04±0.02. Net profit margin results for the different treatments were high for T1 (60) and lowest for T2 (24). The organic fertilizers have significant effects in improving the vegetative growth of plantains therefore, Radix Tim® and DI Grow® were the best treatments for the production of healthy, vigorous nursery plants.
Keywords:
Decortication, Macropropagation, Net profit margin, Organic fertilizers, Plantain plantlet, Vegetative growthDownloads
References
Akongte, P. N., Ntane, O. A., Sumbelle, A. S, Nkengafac, N. J., Nanganoa L. T, & Okolle, N. J. (2019). Comparing the use of a dual-purpose biofertilizer (‘Njoku’) and an inorganic fertilizer on plantain (Musa sp.) growth and infestation in the nursery. Greener Journal of Agricultural Sciences, 9(2), 171-179, http://doi.org/10.15580/GJAS.2019.2.032619051
Al-Harthi, K., Al-Yahyai, R. (2009). Effect of NPK fertilizer on growth and yield of banana in Northern Oman. Journal of Horticulture and Forestry, 1(8),160-167.
Ali, A., Afrasiab, H., Saeed, M., Iqbal, J. (2011). An in vitro study of regeneration and micro- propagation of Mentha arvensis. International Journal of Biology and Biotechnology, 1(4), 519- 528.
Anon (2005). Banana and plantain: A global perspective. Food and Agriculture Organization of the United Nations.
Baiyeri, K. P., & Aba, S.C. (2005). Response of Musa species to macro-propagation. I: Genetic and initiation media effects on number, quality and survival of plantlets at prenursery and early nursery stages. African Journal of Biotechnology, 4, 223-228.
Brukhin, V., & Morozova, N. (2011). Plant growth and development - basic knowledge and current views. Mathematical Modelling of Natural Phenomena, 6(2), 1-53. http://doi.org/10.1051/mmnp/20116201
Cheke, B. L. T., Monono, E., Mih, M. A., & Okolle, J. N. (2020). Comparing the molluscicidal action of metaldehyde, oxamyl, potassium nitrate and a botanical insecticide for managing. International Journal of Sustainable Agricultural Research, 7(3), 143–153. https://doi.org/10.18488/journal.70.2020.73.143.153
Ewané, C. A., Milawé, C. A., Ndongo, E. F., & Boudjeko T. (2020) Influence of clam shells and Tithonia diversifolia powder on growth of plantain PIF seedlings (var. French) and their sensitivity to Mycosphaerella fijiensis. African Journal of Agricultural Research, 15, 393-411. https://doi.org/10.5897/AJAR2019.14486
Ewané, C. A., Ndongo, F., Ngoula, K., Tene-Tayo, P. M., Opiyo, S. O., & Boudjeko, T. (2019) Potential biostimulant effect of clam shells on growth promotion of plantain pif seedlings (var. big ebanga & batard) and relation to Black Sigatoka Disease susceptibility. American Journal of Plant Science, 10, 1763-1788. https://doi.org/10.4236/ajps.2019.1010125
Filipiak, M., & Weiner, J. (2017). Plant– insect interactions: the role of ecological stoichiometry. Acta Agrobotanica, 70(1), 1710. https://doi.org/10.5586/aa.1710
González, J. E., González, A. B., & García, G. C. (2022a). Effects on the plantain proliferation in two propagation environments brought by corm size and benzylaminopurine. Trends in Horticulture, 5(2), 93–101. https://doi.org/10.24294/th.v5i2.1833
González, L. A., Ramírez, M. A., & Rojas, M. I. (2022b). In vitro propagation of plantain (Musa spp.) using the plants from in vitro buds (PIF) technique: A review. Journal of Applied and Natural Science, 14(1), 1-12.
Hassan, S. A. M., Taha, R. A., Zaied, N. S. M., Essa, E. M., & Abd El-Rheem, M. (2022). Effect of vermicompost on vegetative growth and nutrient status of acclimatized Grand Naine banana plants. Heliyon, 8(March), e10914. https://doi.org/10.1016/j.heliyon.2022.e10914
Kubota, C., McClendon, R.W., & Armitage, A.M. (2009). Nitrogen requirements for vegetative growth, flowering, seed production, and postproduction of the nobile dendrobium orchid. HortScience, 44(7), 2027-2033
Kwa, A. K. (2003). The Plants from In Vitro Buds (PIF) technique: A new approach for the mass propagation of plantain (Musa spp.). Plant Cell, Tissue and Organ Culture, 75(3), 233-239.
Kwa, A. K., Tenkouano, A., & Nwaga, D. (2009). In vitro multiplication of plantain (Musa spp.) using the Plants from In Vitro Buds (PIF) technique: A case study of Cameroon. Journal of Applied and Natural Science, 1(1), 1-11.
Lefranc, L. M., Lescot, T., Staver, C., Kwa, M., Michel, I., Nkapnang, I., & Temple, L. (2010). Macropropagation as an innovative technology: Lessons and observations from projects in Cameroon. Acta Horticulturae, (879), 727–733. https://doi.org:10.17660/actahortic
Manju, P. R., & Pushpalatha, P. B. (2020). Macropropagated plantlets in banana: Performance evaluation with suckers and tissue culture plants in Grand Naine and Nendran. Journal of Tropical Agriculture, 58(2), 263–268.
Monono, E. Y., Egbe, E. A., Monono, E. M., Levai, L. D., & Asiedu, E. (2023). Influences of organic waste and inorganic fertilizer for sustainable production of plantain (Musa spp. AAB) in a humid forest zone of Cameroon. African Journal of Agricultural Research, 19(7), 727-742. https://doi.org/10.5897 /AJAR 2023.16349
Monono, E. Y., Ngale, J. E., Dopgima, L. L., & Njukang, A. P. (2018). Use of palm male inflorescence and river-sand as acclimatization substrate for plantain (Musa sp.) cultivars. Biotechnology Journal International, 21(4), 1–10. https://doi.org/10.9734/BJI/2018/43879
Najarian, A., & Souri, M. K. (2020). Influence of sugar cane compost as potting media on vegetative growth, and some biochemical parameters of Pelargonium x hortorum, Journal of Plant Nutrition, 43(17),1-5. https://doi.org/10.1080/01904167.2020.1783305
Nkendah, J., & Akyeampong, K. (2003). The potential of tissue culture technology for banana and plantain production in Africa. African Journal of Biotechnology, 9(23), 3255-3264.
Okolle, J. N., Abuno, M. N., Monono, E. Y., Tatanah, N. & L., Chuyong, G. B. (2022). Integrating Tithonia diversifolia, Piper guineense and oil palm bunch residue ash for the management of sweet potato weevil (Cylas puncticollis Boh.) and on yield of sweet potato in Fako Division of Cameroon. International Journal of Natural Sciences Research, 10(1), 54–73. https://doi.org/10.18488/63.v10i1.3040
Okolle, J. N., Cheke, T. L. B., Monono, E. Y., Ngone, D. M. A., Mih, A. M. (2019). Spatial distribution and seasonal variation of Limicolaria spp. infesting banana plantations in South West, Cameroon. World Journal of Advanced Research and Reviews, 03(02), 36–45. https://doi.org/10.30574/wjarr
Okolle, J. N., Monono, E. Y., Tabikam, A. I., Kinge, M. S., & Pierre Fils Rodrique, M. (2022). Insecticide Use and Application in Cameroon. In Intechopen [Insecticides] (pp. 1–31). https://doi.org/10.5772/intechopen.102634
Okonkwo, U. C., & Nwauzor, S. C. (2021). The use of Plants from In Vitro Buds (PIF) technique in the micropropagation of plantain (Musa spp.) in Nigeria. Journal of Applied and Natural Science, 13(1), 1-12.
Olaghere, I. L., Omotesho, O. A., Ademola, O. E., Osasona, K. K., & Akinrinde, A.F. (2019). Analysis of the profit margin along the plantain value chain in Osun State, Nigeria. Agrosearch, 18(2), 70. https://doi.org/10.4314/agrosh.v18i2.6
Park, Y. S., Kim, J. H., & Kim, H. J. (2011). Nutritional composition and antioxidant properties of banana and plantain. Journal of Food Science, 76(3), C404-C410.
Patel, M. K., & Rath, S. S. (2018). Standardization of macro propagation in banana cultivars - a review. International Journal of Current Microbiology and Applied Sciences, 7(09), 390–400. https://doi.org/10.20546/ijcmas.2018.709.048
Rashid, M. M., Jahan, M., & Islam, K.S. (2016) Impact of nitrogen, phosphorus and potassium on brown plant hopper and tolerance of its host rice plants. Rice Science, 23, 119–131
Tao, C., Li, R., Xiong, W., Shen, Z., Liu, S., Wang, B., Ruan, Y., Geisen, S., Shen, Q., & Kowalchuk, G. A. (2020). Bio-organic fertilizers stimulate indigenous soil pseudomonas populations to enhance plant disease suppression. Microbiome, 8(137), 1–14. https://doi.org/https://doi.org/10.1186/s40168-020-00892-z
Tatsegouock, R. N., Ewané, C. A., & Meshuneke, A. (2020). Plantain Bananas PIF Seedlings treatment with liquid extracts of Tithonia diversifolia induces resistance to Black Sigatoka Disease. American Journal of Plant Sciences, 11, 653–671. https://doi.org/10.4236/ajps.2020.115049

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