Evaluation of antagonistic activities of Bacillus spp. against certain bacteria of medical importance

Abstract

In this work we focused on the antagonistic potential of Bacillus spp. isolates from cow dung. Out of fourteen bacterial strains, isolate KD104 and KD117 were probably identified as Bacillus spp. These two isolates were screened for their antagonistic activity against 14 test organisms viz., Vibrio Cholera (MTCC 3904), Salmonella typhi (MTCC 3216), Escherichia coli (SGPGI), Staphylococcus aureus (MTCC 7443), Bacillus subtilis (MTCC 441), Bacillus cereus (MTCC 6728), Proteus vulgaris (MTCC 426), Enterococcus faecalis (MTCC 439), Pseudomonas aeruginosa (MTCC 424), Escherichria coli (MTCC 118), Shigella flexneri (MTCC 1457), Salmonella typhimurium (MTCC 3231), Streptoccus pyogenes (MTCC 442) and Staphylocccus aureus (MTCC 3160) using cross-streak method. The preliminary screening revealed significant antimicrobial effect of both isolates against Vibrio Cholera (MTCC 3904), Salmonella typhi (MTCC 3216), Escherichia coli (SGPGI), and Bacillus cereus (MTCC 6728). Therefore, this study indicates that these Bacillus species may be up-hold to industrial level for production of antimicrobial agent, which should be further analyzed for its possibility to be used as therapeutic agent.