Blast resistance (Pi54) introgression in temperate rice (Oryza sativa L.) K343 using marker assisted backcrossing
https://doi.org/10.26832/24566632.2023.080109
Abstract
In hill rice ecologies, Magnaporthe oryzae causes rice blasts and a significant biotic constraint. The study was aimed to develop rice blast-resistant lines/varieties through marker-assisted selection (MAS) by using the Pi54 gene, which provides resistance against the most prominent blast fungus isolate (PLP-1). The blast resistance gene Pi54 from the indica rice genotype DHMAS has been inserted into the genetic makeup of the temperate rice variety K343 in the current study. Three SSR markers (TRS26, TRS33, and RM206) are closely linked to the Pi54 locus used in this study. Marker RM206, located 0.7cM from the Pi54 gene, distinguished between donor and recipient alleles and co-segregated with the target gene. Thus, RM206 is used for Pi54 gene foreground selection. Sixty-one plants have the homozygous allele for the Pi54 in BC1F2, accounting for roughly 50% of the homozygous population. Polymorphic genome-wide SSR assessment of the BC1F2 genetic stock (K343*2/DHMAS) revealed recurrent parent genome (RPG) recovery above 75% in 4 plants. Therefore, M. oryzae race PLP-1, the dominant race used in this study, showed high resistance to the resistant response after inoculation.
Keywords:
Magnaporthe oryzae, Marker-Assisted Backcrossing, Pi54, Rice blast, RPGDownloads
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