Prevalence of antibodies against classical swine fever in two districts of Nepal
Abstract
This study aimed to investigate the seroprevalence of Classical Swine Fever (CSF), caused by the Classical Swine Fever Virus (CSFV), in the Bhaktapur and Kavrepalanchok districts of Nepal. The primary objective was to determine the prevalence of antibodies against CSFV in the swine population, providing crucial insights for targeted interventions and control strategies. A cross-sectional study was conducted between January and March 2018. The research involved structured questionnaire surveys and blood sample collection from selected farms in the study area. A total of 184 serum samples were collected and subjected to testing using the IDEXX ELISA test kit. The study evaluated seroprevalence by considering variables such as district, sex, age groups, and housing systems. Out of the 184 serum samples collected, 34 were found to be seropositive for CSFV antibodies, resulting in an overall seroprevalence of 18.40%. The study identified significant variations in seroprevalence between different factors, including districts, sexes, age groups, and housing systems. These findings indicate a notable exposure of swine populations in the Bhaktapur and Kavrepalanchok districts to CSFV. This research highlights the considerable risk posed by CSF to the pig populations in the Bhaktapur and Kavrepalanchok districts of Nepal. The identified seroprevalence and variations among different factors emphasize the importance of targeted interventions and control strategies in these regions. The findings serve as a foundation for informed decision-making to mitigate the impact of CSF, safeguard swine health, and protect the livelihoods of those dependent on the swine industry. This study was made possible with the support of the Zoonosis Control Project under the National Animal Science Research Institute, Nepal Agriculture Research Council.
Keywords:
Classical swine fever, CSFV, IDEXX ELISA test kit, Nepal, SeroprevalenceDownloads
References
Brookes, V. J., Barrett, T. E., Ward, M. P., Roby, J. A., Hernandez-Jover, M., Cross, E. M., & Diseases, E. (2021). A scoping review of African swine fever virus spread between domestic and free‐living pigs. Transboundary and Emerging Diseases, 68(5), 2643-2656.
Chaudhary, J., Singh, N. S., Tolenkhomba, T., Behera, S., & Vanlalchhuanga, V. J. I. J. O. A. S. (2019). Estimation of economic losses due to classical swine fever in pigs in Mizoram. Indian Journal of Animal Sciences, 89(3), 229-232.
Council, N. R. (2005). Animal health at the crossroads: preventing, detecting, and diagnosing animal diseases. National Academies Press.
Deka, D., Barman, N. N., Deka, N., Batth, B. K., Singh, G., Singh, S., & Production. (2021). Sero-epidemiology of porcine parvovirus, circovirus, and classical swine fever virus infections in India. Tropical Animal Health and Production, 53, 1-12.
Dione, M., Masembe, C., Akol, J., Amia, W., Kungu, J., Lee, H. S., & Wieland, B. J. A. t. (2018). The importance of on-farm biosecurity: Sero-prevalence and risk factors of bacterial and viral pathogens in smallholder pig systems in Uganda. Acta Tropica, 187, 214-221.
Edwards, S., Fukusho, A., Lefevre, P. C., Lipowski, A., Pejsak, Z., Roehe, P., & Westergaard, J. J. V. m. (2000). Classical swine fever: the global situation,Veterinary Microbiology, 73(2-3), 103-119.
Eshima, N., Tokumaru, O., Hara, S., Bacal, K., Korematsu, S., Tabata, M., & Matsuishi, T. J. P. O. (2011). Sex-and age-related differences in morbidity rates of 2009 pandemic influenza A H1N1 virus of swine origin in Japan. Plos One 6(4), e19409.
Gao, L., Zhang, L., Xu, H., Zhao, F., Ke, W., Chen, J., & Liu, J. J. V. M. (2020). The Actinobacillus pleuropneumoniae sulfate-binding protein is required for the acquisition of sulfate and methionine, but is not essential for virulence. Veterinary Microbiology, 245, 108704.
Gladue, D. P., Gavrilov, B. K., Holinka, L. G., Fernandez-Sainz, I. J., Vepkhvadze, N., Rogers, K., & Borca, M. V. J. V. (2011). Identification of an NTPase motif in classical swine fever virus NS4B protein. Virology, 411(1), 41-49.
Greiser-Wilke, I., Blome, S., & Moennig, V. J. V. (2007). Diagnostic methods for detection of Classical swine fever virus—status quo and new developments. Vaccine,25(30), 5524-5530.
Greiser-Wilke, I., & Moennig, V. J. A. H. R. R. (2004). Vaccination against classical swine fever virus: limitations and new strategies. Animal Health Research and Reviews,5(2), 223-226.
Moennig, V., Floegel-Niesmann, G., & Greiser-Wilke, I. J. T. V. J. (2003). Clinical signs and epidemiology of classical swine fever: a review of new knowledge. The Veterinary Journal, 165(1), 11-20.
Moennig, V. J. A. H. R. R. (2004). Vaccination against classical swine fever virus: limitations and new strategies. Animal Health Research and Review, 5(2).
Postel, A., Nishi, T., Kameyama, K.-i., Meyer, D., Suckstorff, O., Fukai, K., & Becher, P. J. E. I. D. (2019). Reemergence of classical swine fever, Japan, 2018. Emerging Infectious Disease, 25(6), 1228.
Postel, A., Schmeiser, S., Bernau, J., Meindl-Boehmer, A., Pridotkas, G., Dirbakova, Z., & Becher, P. J. V. R. (2012). Improved strategy for phylogenetic analysis of classical swine fever virus based on full-length E2 encoding sequences. Veterinary Research, 43, 1-15.
Schwartz, W., & Smallwood, J. J. N. Z. V. J. (1977). Collection of blood from swine. New Zealand Veterinary Journal, 25(9), 237-238.
Stroh, E., Fischer, K., Schwaiger, T., Sauerhering, L., Franzke, K., Maisner, A., & Diederich, S. J. V. m. (2019). Henipavirus-like particles induce a CD8 T cell response in C57BL/6 mice. Veterinary Microbiology, 237, 108405.
Verzani, J. (2011). Getting started with RStudio. " O'Reilly Media, Inc.".
Villarta, Jr, R. L., & Asaad, A. S. J. A. M. P. (2014). Sample size determination in an epidemiologic study using the EpiTools web-based calculator. Acta Medica Philippina, 48(1).
Yu, S., Yin, C., Song, K., Li, S., Zheng, G.-L., Li, L.F., & Sun, Y. J. J. O. G. V. (2019). Engagement of cellular cholesterol in the life cycle of classical swine fever virus: Its potential as an antiviral target. Transboundary and Emerging Diseases, 100(2), 156-165.
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