In-vitro evaluation of antifungal effects of botanical extracts against Colletotrichum lindemuthianum causing anthracnose of beans

Netra Prasad Pokharel 1 , Prashant Gyanwali 2 , Bhuwan Tharu 3 , Sandesh Paudel 4 , Renuka Khanal 5 , Rajan Paudel 6

1   Institute of Agriculture and Animal Science (IAAS), Tribhuwan University (TU), Paklihawa, Rupandehi, NEPAL
2   Institute of Agriculture and Animal Science (IAAS), Tribhuwan University (TU), Paklihawa, Rupandehi, NEPAL
3   Institute of Agriculture and Animal Science (IAAS), Tribhuwan University (TU), Paklihawa, Rupandehi, NEPAL
4   Institute of Agriculture and Animal Science (IAAS), Tribhuwan University (TU), Paklihawa, Rupandehi, NEPAL
5   Institute of Agriculture and Animal Science (IAAS), Tribhuwan University (TU), Paklihawa, Rupandehi, NEPAL
6   Institute of Agriculture and Animal Science (IAAS), Tribhuwan University (TU), Paklihawa, Rupandehi, NEPAL

✉ Coressponding author: See PDF.




Anthracnose of beans caused by Colletotrichum lindemuthianum is regarded as one of the most damaging diseases of legumes, producing major losses in productivity and quality every year. Several fungicides are available for control but are dangerous to the health of the environment and organisms, so developing organic solutions is imperative. The in-vitro study includes five botanicals namely neem, mugwort, garlic, ginger, and wild sage, to evaluate their capacity to inhibit the mycelial growth of C. lindemuthianum. Analysis was done with the poisoned food technique with four replications for each botanical used in a completely randomized design (CRD). The maximum growth inhibition (100%) was observed in garlic at 10% concentration, followed by ginger (81.15%), while the minimum growth inhibition (36.58%) was observed in wild sage at 10% concentration. Garlic, neem, and mugwort exhibited greater efficiency at lower concentrations, whereas ginger and wild sage demonstrated increased efficiency with higher concentrations. The in-vitro study needs to be tested in field conditions to determine the practical efficiency of the botanicals, and a focus should be made on using garlic and ginger to find organic solutions against the anthracnose of beans.


Botanical extracts, Colletotrichum, Colony inhibition, Fungicide, Poisoned food technique


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How to Cite

Pokharel, N. P., Gyanwali, P., Tharu, B., Paudel, S., Khanal, R., & Paudel, R. (2024). In-vitro evaluation of antifungal effects of botanical extracts against Colletotrichum lindemuthianum causing anthracnose of beans. Archives of Agriculture and Environmental Science, 9(2), 201-206.



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