Transcriptional activator gene based phylogenetic analysis of dolichos yellow mosaic virus infecting lablab bean (Dolichos lablabL.)

Abstract

Lablab bean (Dolichos lablab L.) is one the important crop, cultivated as vegetable, pulses as it is rich in protein. It is affected from viral disease i.e. dolichos yellow mosaic virus. The causative agent is begomovirus belongs to geminivirus family. Begomovirus contain bipartite genome having two type of DNA-A and DNA-B. DNA- A helps in replication and DNA-B helps in movement. These DNA have six different type of gene coat protein gene, transcriptional activator gene, replica-tion associated gene, replication enhancer gene, pre coat protein involved in different function associated with it. The present investigation was carried out to investigate the transcriptional activa-tor gene based phylogenetic analysis of Dolichos yellow mosaic virus infecting D. lablab. This study is based on the transcriptional activator gene which is used in transactivation of genes, contains three conserved domains: a basic domain at N-Terminus, a central DNA binding domain and activator domain. The genome databases of dolichos yellow mosaic virus were taken from NCBI site total six genome was available and were used with Clustal W and CLC BIO were the bioinformatic tools for determining sequence homology among genome present in different geographical location. The absence of functional specificity suggests that all begomovirus contains a common element interacts with cellular proteins of other viruses reveals the phylogenetic analysis with the other species Dolichos in the different geographical location.