Impact of Rhizobium biofertilizer on agronomical performance of lentil (BARI Masur-6) in Bangladesh
https://doi.org/10.26832/24566632.2021.060201
Abstract
Excessive nitrogen fertilizer uses in crop field causes surface water pollution, which has a harmful effect on the ecosystem. The study was conducted to reducing the use of nitrogen fertilizers during cultivation of pulse crop and as an alternative to increase the use of biofertilizers. Rhizobium can fix atmospheric nitrogen to the soil; it can be used as an alternative to urea for the cultivation of lentil (BARI Masur-6). The Rhizobium leguminosarum was isolated from root nodules of lentil (Lens culinaris) plants and cultured in YEMA (Yeast Extract Mannitol Agar) media. The Rhizobium was screened on the ground of physiological, biochemical and environmental conditions. Different doses of urea fertilizers (20, 40, 60, 80 kg ha-1) and liquid Rhizobium were used in experimental plots. The results indicated that biofertilizer with different chemical fertilizer performed higher than application of several level of urea nitrogen fertilizer in respect of plant height, number, chlorophyll content (µg cm-1) and number of nodules plant-1 with variety BARI Masur-6. There was optimum relative growth rate (RGR) also observed. The increase in urea nitrogen levels was the reason for the decline in relative plant growth. Yield and yield contributing characters like number of pod plant-1, number of seed plant-1, 1000-grain weight (g), grain yield (t ha-1), straw yield and biological yield were significantly influenced by biofertilizer application. The assembled application of biofertilizer and chemical fertilizer produced maximum number of harvest index (%) compare to the chemical fertilizers. Significant correlation found with no of nodule, no of seed, seed weight, grain yield and straw yield. Significant correlation also found in chlorophyll content with some yield contributing characters like seed number, seed weight, grain yield and straw biomass. Further significant correlation observed between pod number and seed number, between seed weight and grain yield. Rhizobium can fulfill the alternate source of nitrogen that promoted significant growth and yield of lentil and it was much closer to the farmer’s conventional amount of urea.
Keywords:
Biofertilizer, Nitrogen, Root nodule, Rhizobium, SymbiosisDownloads
References
Abo-Hegazy, S. R. E., Selim, T., & El-Emam, E. A. A. (2012). Correlation and path coefficient analysis of yield and some yield components in lentil. Egyptian Journal of Plant Breeding, 16(3), 147-159.
Ansari,J., Sharma, C. P., Sagar, A. and Fatma A.(2015). Interactive effect of bio-fertilizers (Rhizobium, PSB and VAM) in lentil (Lens culinaris L.). Research in Environment and Life Sciences, 8(4), 613-614.
Asghar, A. B., Roider, K.B., & Keating, J. H., (1988). Effects of inoculation and phosphate fertilizer on lentil under rainfed conditions in upland Baluchistan (Lens culinaris). Lens Newsletter, 15(1), 29-33.
Bagher, A., Khosro, A., Saeed, H., & Mohammad, S. H. (2013). Regression modeling of growth indices of Lentil affected by Bio-fertilizers with Superabsorbent polymer. International Journal of Farming and Allied Sciences, 2(19), 712-719.
Begum, S., & Begum, S. (1996). Morphological study and character association in germplasm of lentil (Lens culinaris Medik). Bangladesh Journal of Botany, 25, 79-82.
Bhuiyan, M. A. H., Islam, M. S., & Ahmed, M. S. (2015). Response of lentil to bio and chemical fertilizers at farmers field. Bangladesh Journal of Agricultural Research, 40(3), 501-506.
Das, T., Mahapatra, S. & Das, S. (2017). In vitro Compatibility Study between the Rhizobium and Native Trichoderma Isolates from Lentil Rhizospheric Soil. International Journal of Current Microbiology and Applied Sciences 6(8): 1757-1769.
Date, R. A. & Halliday, J. (1987). Collection, isolation, cultivation and maintenance of rhizobia.In Elkan, G.H., Ed. Symbiotic Nitrogen Fixation Technology. Marcel Dekker, New York, NY, pp. 1-27
Ezzat, Z. M., & Ashmawy, F. (1999). Performance of some exotic lentil genotypes under Egyptain conditions. Zigzag Journal of Agricultural Research, 26, 267-280.
Fikiru E, Tesfaye K, Bekele E. (2007). Genetic diversity and population structure of Ethiopian lentil (Lens culinarisMedikus) landraces as revealed by ISSR marker. African Journal of Biotechnology, 6(12):1460–1468.
Gardner, F. P., Pearce, R. B., & Mitchell, R. L. (1985). Physiology of Crop Plants. Iowa State University Press.
Gomare K.S., Mese M. and Shetkar Y. (2013). Isolation Rhizobium and cost effective production of biofertilizer. Indian Journal of Life Sciences, 2(2):49-53.
Hoque, M. M., & Haq, M. F. (1994). Rhizobium inoculation and fertilization of lentil in Bangladesh. Lens Newletter, 21, 29-30.
Hossain, B. M. (2018). Effects of Rhizobial strains and urea fertilizer on lentil at different locations of Bangladesh. Bangladesh Journal of Botany, 47(2), 181-185.
Hossain, M. M., & Suman, M. (2005). Effect of biofertilizer and nitrogenous fertilizer on growth, yield and N-uptake of lentil (Lens culinaris Medik). International Journal of Agricultural Technology, 1(6), 76-81.
Htwe, A. Z., Moh, S. M., Moe K. and Yamakawa T. (2019). Biofertilizer Production for Agronomic Application and Evaluation of Its Symbiotic Effectiveness in Soybeans. Agronomy 9, 162.
Idris, M., Tariq, M., & Malik, K. A. (1989). Response of field-grown chickpea (Cicer arietinum L.) to phosphorus fertilization for yield and nitrogen fixation. Plant and Soil, (114), 135-138.
Jain, S. K., Sharma, H. L., Mehra, R. B., & Khare, J. P. (1991). Multiple correlation and regression analysis in lentil. LENS, 18, 11-23.
Jessop, R. S., Sparke, M. C., & Sale, P. W. G. (1989). Phosphorus fertilizer response in narrow leafed lupin (Lupinus angustifolius L.) and chickpea (Cicer arietinum) compared to wheat (Triticum aestivum) in early growth. Fertilizer Research, (19), 1-6.
Juwarkar, A. S., Juwarkar, A., & Khanna, P. (2004). Use of selected waste materials and biofertilizers for industrial solid waste reclamation. In Waste Management Series (Vol. 4, pp. 911-948). Elsevier.
Khanam, D., Bhuiyan, M. A. H., Rahman, M. H. H., & Hossain, A. K. M. (1999). On-farm experience of the application and adoption of biological nitrogen fixation technology in Bangladesh. Bangladesh Journal of Agricultural Research, 24(2), 375-382.
Khanam, D., Rahman, M. H. H., Bhuiyan, M. A. H., Hossain, A. K. M., & Rahman, A. F. M. (1993). Effect of rhizobial inoculation and chemical fertilizer on the growth and yield of lentil at Two Agroecological zones of Bangladesh. Bangladesh Journal of Agricultural Research, 18(2), 196-200.
Kumar, V., Kumar, P., & Singh, J. (2019). An introduction to contaminants in agriculture and environment. Contaminants in Agriculture and Environment: Health Risks and Remediation, 1, 1-8.
Luthra, S. K., & Sharma, P. C. (1990). Correlation and path analysis in lentils. Lens Newsletter, 17, 5-8.
Nadeem, M. A., Ahmad, R., & Ahmad, M. S. (2004). Effects of seed inoculation and different fertilizers levels on the growth and yield of mungbean. Journal of Agronomy, 3(1), 40-42.
Podder, A. K., Satter, M. A., & Marshed, G. (1989). Performance studies of some isolated native Rhizobial inocula on the production of lentil with or without nitrogen fertilizer. Proc. 6thNational Botany Conference January 18-19 Abstract No. 43, 23.
Poonia, S. (2011). Rhizobium: A Natural Biofertilizer. Department of Botany, D.N. College, Meerut, U.P, INDIA International Journal of Engineering and Management Research, 191, 36-38.
Prell, J., & Poole, P. (2006). Metabolic changes of rhizobia in legume nodules. Trends in Microbiology, 14, 161-168.
Rajeshwari, P., Aishwaryaalakshmi, B., & Jeyagowri, C. (2017). Isolation, identification and screening of Rhizobium for plant growth promotion. International Journal of Applied Research, 3(1), 732-733.
Rajput, M.A., & Sarwar, G. (1989). Genetic variability, correlation studies and their implication in selection of high yielding genotypes in lentil. Lens Newsletter, 16, 5-8.
Saraf, C. S., Patil, R. R., & Prashad, M. (1985). Correlation and regression studies in lentil cultivars. Lens Newsletter, 12, 11-22.
Sarker, A., & Kumar S. (2011) Lentils in production and food systems in West Asia and Africa. International Center for Agricultural Research in the Dry Areas (ICARDA), Aleppo, Syria. Grain Legumes. 57, 46–48.
Sekhon, H. S., Singh, G., Sharma, P., & Sharma, A. K. (2002). Effects of Rhizobium inoculation and nitrogen on biological nitrogen fixation, growth and yield of mungbean. Environment and Ecology, 20, 282-290.
Senanayake, L., Knieve, l. D. P., & Stevena, S. E. (1987). Nodulation and symbiotic nitrogen fixation of cowpea (Vigna unguiculata L.). Plant and Soil, (99), 435-439.
Somasegaran, P., & Hoben, H. J. (1994). Handbookof Rhizobia. Methods in Legume-Rhizobium Technology. Springer-Verlag, New York, NY,pp. 450.
Sultan, M. M. (1993). Influence of Rhizobium inoculants and urea nitrogen on
the yield and yield contributing characters of lentil (Lens culinaris Meidk). MS Thesis. Dept. of Soil Science. Bangladesh Agricultural University, Mymensingh.
Thimmaiah, S. K. (1999). Standard methods of biochemical analysis. Kalyani Publishers, Ludhiana, 534.
Viviene, N. M., & Dakora, F. D. (2004). Potential use of rhizobial bacteria as promoters of plant growth for increased yield in landraces of African cereal crops. African Journal of Biotechnology, 3(1), 1-7.
Yahiya, M., Samiullah, & Futma A. (1995). Influence of phosphorus on nitrogen fixation in chickpea cultivars. Journal of Plant Nutrition, (18), 719-727.
Published
How to Cite
Issue
Section
Copyright (c) 2021 Agriculture and Environmental Science Academy
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
