Isolation and identification of Azotobacter from saline and non-saline soils of Bangladesh
https://doi.org/10.26832/24566632.2025.100102
Abstract
Nitrogen is the most important mineral nutrient required for the plants growth and development. Microbial inoculants have the potential to augment and reduce reliance on expensive chemical fertilizers specially Urea, while maintaining crop productivity. Urea fertilizer not only expensive but also destroy our environment by nitrate pollution. Some microbes have ability to fix atmospheric nitrogen in soil symbiotically in legume crops. However, there are a few reports on non-symbiotic nitrogen fixers for non- leguminous crops. The present study aimed to isolate efficient non symbiotic or free living Azotobacter spp. that could be utilized as potential bio inoculants for resolving the nitrogen deficiency in soil for better growth and development of non-leguminous crops as well as industrial benefits. Nine Azotobacter isolates from saline and non-saline soils of south coastal zones of Bangladesh cultured on Ashby’s agar plates at 28oC for 1 week. The colonies on the medium were picked up and used for the investigations. Based on morphological and biochemical identifications the isolates were confirmed as Azotobacter spp., isolates showed higher nitrogen fixing abilities (6.95 to 9.89 mg N/g) were selected. Among them, isolate NFA3 performed the best (9.89 mg N/g) regardless of all the tests. These isolates could survive neutral to slightly saline soils and higher temperature. Therefore, isolate NFA3 was considered to be the candidate for industrial usage for the development of nitrogenous biofertilizer.
Keywords:
Azotobacter, Biofertilizer, Nitrogen fixing bacteriaDownloads
References
Alshibli, N. A. (2018). Diversity of Free – Living Nitrogen – Fixing Bacteria in Soil of Sioux Prairie of South Dakota. Electronic Theses and Dissertations, 2959. https://openprairie.sdstate.edu/etd/2959
Amarger, N., Bours, M., Revoy, F., Allard, M. R., & Laguerre, G. (1994). Rhizobium tropicinodulates field-grown Phaseolus vulgaris in France. Plant and Soil, 161(2), 147–156. http://www.jstor.org/stable/42939498
Bhardwaj, D., Ansari, M. W., Sahoo, R. K., & Tuteja, N. (2014). Biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity. Microbial Cell Factories, 13, 66, https://doi.org/10.1186/1475-2859-13-66
Chen, J. (2006). The combined use of chemical and organic fertilizers and/or biofertilizer for crop growth and soil fertility. International workshop on Sustained Management of the Soil–Rhizosphere System for Efficient Crop Production and Fertilizer Use, Thailand, 1–10.
Dahal, B., NandaKafle, G., Perkins, L., & Brözel, V. S. (2017). Diversity of free-Living nitrogen-fixing Streptomyces in soils of the badlands of South Dakota. Microbiology Research, 195, 31-39.
Deschodt, C. C., & Strijdom, B. W. (1976). Effective Nodulation of Aspalathus Linearis Ssp. Linearis Byrhizobiafrom other Aspalathus Species. Phytophylactica, 8, 103-104.
Franche, C., Lindstrom, K., & Elmerich, C. (2009). Nitrogen fixing bacteria associated with leguminous and non-leguminous plants. Plant and Soil, 321, 35-59.
Hossain, M. M., Akter, S., Hasan, M. M., Hasan, A., Uddin, K. R., Parvin, A., Jahan, I., Rahman, M. N., & Rahman, S. M. B. (2014). Nitrogen fixing efficiency and physiological characteristics of Azospirillum isolates from the paddy fields of North Bengal, Jahangirnagar University Journal of Biological Science, 3, 47-53.
Kucuk, C., Kivanc, M., & Kinac, E. (2006). Characterization of Azotobacter Sp. Isolated from Bean. Turkish Journal of Biology, 30, 127-132.
Lenart, A. (2012). Occurance Characteristics and Genetic Diversity of Azotobacter chroococcum in Various Soils of Southern Poland. Polish Journal of Environmental Studies, 21(2), 415-424.
Nagananda, G. S., Das, A., Bhattachrya, S., & Kalpana, T. (2010). In vitro studies on the Effects of Biofertilizers (Azotobacter and Rhizobium) on Seed Germination and Development of Trigonella foenum–graecum L. using a Novel Glass Marble containing Liquid Medium. International Journal of Botany, 6(4), 394–403.
Sant'Anna, F. H., Almeida, L. G. P., Cecagno, R., Reolon, L. A., Siqueira, F. M., Machado, M. R. S., Vasconcelos, A. T. R., & Schrank, I. S. (2011). Genomic insights into the versatility of the plant growth-promoting bacterium Azospirillum amazonense. BMC Genomics, 12, 409, https://doi.org/10.1186/1471-2164-12-409
Shraddha, B. Dr. R. V., Vyas, H., Shelat, N., & Sneha, J. (2013). Isolation and Identification of Root Nodule Bacteria of Mung Bean (Vigna radiate L.) for Biofertilizer Production. International Journal of Research in Pure and Applied Microbiology, 3(4), 127-133.
Somasegaran, P., & Hobben, H. J. (1985). Methods in legume-Azotobacter technology. NifTAL project and MIRCEN, Department of Agronomy and Soil science, University of Hawaii.
Vincent, J. M., Nutman, P. S., & Skinner, F. A. (1980). Some General Techniques and Procedures-Identification and Classification of Azotobacter. Research for development seminar on "Nitrogen Fixation by Legumes for Tropical Agriculture" held in Canberra. Australia, during Nov.-Dec. 1980.
Vitousek, P. M. (1997). Human alteration of the global nitrogen cycle: sources and consequences. Ecological Applications, 7, 737-750.
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