In vitro propagation of two strawberry (Fragaria ananassa Duch.) cultivars of Bangladesh

Abstract

An experiment on in vitro propagation of strawberry was carried out at Plant Breeding and Biotechnology Laboratory of Agrotechnology Discipline, Khulna University, Khulna, Bangladesh during the period of February to November, 2015. In the experiment, runners of two strawberry cultivars viz. BARI Strawberry^-1 and Modern Strawberry-5 (Festival) were cultured on MS  media supplemented with different concentrations of BAP (1.0, 1.5 and 2.0 mgL^-1) and Kin (0.1, 0.5 and 1.0 mgL^-1) for multiple shoot regeneration. The experiment was laid out in Completely Randomized Design (CRD) with three replications. The highest number of shoots (10.41) was obtained from Modern Strawberry-5 and maximum shoot length (13.62 mm) obtained from BARI Strawberry-1. Maximum number of shoots (13.33) and shoot length (24.29 mm) were recorded from MS media containing 1.0 mgL^-1 BAP and 1.0 mgL^-1 Kin. Then all the regenerated plantlets were cultured on MS media containing 2.0 mgL^-1 IAA for root initiation. Greater reduction in vigor was observed in Modern Strawberry-5 (Festival) when they were transferred to root induction media. Ex vitro survivability of the complete plantlets varied from 36.67 to 6.67%. Maximum survivability of both varieties 36.67% (BARI Strawberry^-1) and 26.67% (Festival) were recorded from MS media supplemented with 1.0 mgL-1 BAP and 1.0 mgL^-1 Kin. For in vitro micropropagation, BARI Strawberry-1 may be better and MS media supplemented with 1.0 mgL^-1 BAP and 1.0 mgL^-1 Kin may be better.